Molecular Profiling
In order to gain a better understanding of the molecular basis of head and neck cancer (HNC), and derive effective markers for the development of better diagnostic approaches and treatments with less toxicity and longer survival, the Liu lab has utilized high-throughput screening tools to investigate the genetic aberrations of the entire cancer genome.
Recent advancements in high resolution array-based CGH allows for the detection of micro-regions of copy number aberrations by hybridizing differentially labeled test (tumor) and reference genomes to arrays of spotted BAC clones. The results can be used to construct high-resolution maps of amplifications and deletions regions. Moreover, the advent of microarray technology has revolutionized the study of molecular pathways, which are responsible for the development and progression of human malignancies. The Liu lab has had a long-time interest in developing molecular therapies for HNC. To explore novel potential therapeutic targets and to attain a better understanding of the pathways involved in HNC development, we have also performed gene expression profile studies using primary human HNC biopsy samples.
By integrating the measurement of whole tumor genome copy number and comprehensive transcription profile, we have identified important cancer related genes. These genes can then be used as molecular genetic markers for diagnosis, prognosis, and monitoring of disease progression, novel target(s) for the development of new anti-cancer drugs and design of treatment protocols.
Comparison of the pattern of gene expression between normal and NPC biopsy specimens.
Representative Publications:
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